DataSets

rRNA secondary structures allow organization and illustration of three-dimensional architecture, local molecular interactions, phylogeny, evolution, and function. Our goal is to represent secondary structures that are as accurate and useful as possible, using 3D crystal structures, or full atom models into good Cyro-EM models, ribosomes as input. We offer secondary structures for 23S/28S/5S and 16S/18S rRNAs of E. coli, Thermus thermophilus, Haloarcula marismortui, Saccharomyces cerevisiae, Drosophila melanogaster, and Homo sapiens. Both the large and small subunit are available for each species, except the small subunit for Haloarcula marismortui. All species have a 3D-based secondary strucutre available. Some species also have a classic phylogeny-based structure available. Additionally, we provide high-resolution editable versions of those structures mapped with a variety of data related to molecular interactions and geometry, phylogeny and evolution, and partitioning of rRNA into helices and domains (http://apollo.chemistry.gatech.edu/RibosomeGallery).

Scientific Credits

Credits for the new 3D-based Secondary Structures

Petrov et al., (2013) "Secondary Structure and Domain Architecture of the 23S rRNA", Nucleic Acids Res. doi: 10.1093/nar/gkt513

Credits for the Secondary Structures

http://www.rna.icmb.utexas.edu/CAR/1A/

http://rna.ucsc.edu/rnacenter/ribosome_images.html

Woese C R, Magrum L J, Gupta R, Siegel R B, Stahl D A, Kop J, Crawford N, Brosius J, Gutell R, Hogan J J and Noller H F 1980 Nucleic Acids Res. 8 2275

Noller H F, Kop J, Wheaton V, Brosius J, Gutell R R, Kopylov A M, Dohme F, Herr W, Stahl D A, Gupta R and Woese C R 1981 Nucleic Acids Res. 9 6167

Noller H F 1984 Annual Review of Biochemistry 53 119

Gutell RR, Weiser B, Woese CR, and Noller HF 1985 Prog. Nucleic Acids Res. Mol. Biol. 32 155

Gutell R R and Fox G E 1988 Nucleic Acids Research 16 R175

Gutell R R, Larsen N and Woese C R 1994 Microbiol Rev 58 10

Cannone J J, Subramanian S, Schnare M N, Collett J R, D'Souza L M, Du Y, Feng B, Lin N, Madabusi L V, Muller K M, Pande N, Shang Z, Yu N and Gutell R R 2002 BMC Bioinformatics 3 2

Ben-Shem A, Jenner L, Yusupova G and Yusupov M 2010 Science 330 1203

Anger, A. M.; Armache, J. P.; Berninghausen, O.; Habeck, M.; Subklewe, M.; Wilson, D. N.; Beckmann, R.. Nature 2013, 497 (7447), 80-5.

Credits for the X-ray (or Cryo-EM) Structures

Yusupov M M, Yusupova G Z, Baucom A, Lieberman K, Earnest T N, Cate J H and Noller H F 2001 Science 292 883

Ban N, Nissen P, Hansen J, Moore P B and Steitz T A 2000 Science 289 905

Dunkle J A, Wang L Y, Feldman M B, Pulk A, Chen V B, Kapral G J, Noeske J, Richardson J S, Blanchard S C and Cate J H D 2011 Science 332 981

Ben-Shem A, de Loubresse N G, Melnikov S, Jenner L, Yusupova G and Yusupov M 2011 Science 334 1524

Anger, A. M.; Armache, J. P.; Berninghausen, O.; Habeck, M.; Subklewe, M.; Wilson, D. N.; Beckmann, R.. Nature 2013, 497 (7447), 80-5.

Credits to SILVA for Sequences and Alignment

Pruesse E, Peplies J and Glockner F O 2012 Bioinformatics 28 1823

Credits to FR3D for Interaction Data

Sarver M, Zirbel C L, Stombaugh J, Mokdad A and Leontis N B 2008 J Math Biol 56 215